Last data update: May 06, 2024. (Total: 46732 publications since 2009)
Records 1-7 (of 7 Records) |
Query Trace: Kelly AJ[original query] |
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Using the BDFX40 Automated Continuous Blood Culture System to Isolate and Recover Streptobacillus moniliformis in the Presence of 0.05% SPS: A 55-Year, 56-Strain Retrospective Study
Szewc AM , Bell ME , Kelly AJ , Humrighouse BW , McQuiston JR . Lab Med 2021 52 (6) 536-549 Rat bite fever and Haverhill fever are often difficult to diagnose in a clinical setting. This difficulty results in part from clinicians and laboratory professionals not being able to reliably recover the causative agent Streptobacillus moniliformis using culture-based methods. After utilizing an automated continuous-monitoring blood culture bottle system, we showed that the organism can be reliably cultured when a blood volume inoculum of 10 mL is used. Further, we showed that when the above recommendation is followed, sodium polyanethole sulfonate (up to a concentration of 0.05% w/v) in commercially purchased blood culture bottle formulations seems to be inactivated, allowing for the growth and detection of S. moniliformis. Herein, we offer data and methods used to overcome these clinical limitations. This is a comprehensive study of the historical collection of S. moniliformis isolates maintained by our facility and believed to be the largest of its kind to date. |
A real-time multiplex PCR assay for detection of the causative agents of rat bite fever, Streptobacillus moniliformis and zoonoticStreptobacillus species.
Kelly AJ , Ivey ML , Gulvik CA , Humrighouse BW , McQuiston JR . Diagn Microbiol Infect Dis 2021 100 (2) 115335 Rat bite fever (RBF) caused by Streptobacillus moniliformis has been described as a diagnostic challenge. While it has a favorable prognosis with treatment, timely diagnosis is hindered by the lack of culture-free identification methods. Here we present a multiplex real-time PCR assay that detects the zoonotic Streptobacillus spp. as well as differentiate the primary causative agent of RBF, Streptobacillus moniliformis. The performance of this assay was evaluated using mock clinical specimens for blood, serum, and urine. Analytical sensitivity was determined to be 3-4 genome equivalents (GE)/µl for the zoonotic Streptobacillus spp. target, and 1-2 GE/µl for the S. moniliformis specific target. The assay correctly detected only the intended targets with no cross-reactivity identified. The pathogen was detected in all spiked matrices and not detected in the negative non-spiked specimens. This rapid diagnostic assay may permit quicker diagnosis of RBF patients. |
A real-time multiplex PCR assay for detection of Elizabethkingia species, and differentiating between E. anophelis and E. meningoseptica .
Kelly AJ , Karpathy SE , Gulvik CA , Ivey ML , Whitney AM , Bell ME , Nicholson AC , Humrighouse BH , McQuiston JR . J Clin Microbiol 2019 57 (4) Nosocomial infections of Elizabethkingia species can have fatal outcomes if not identified and treated properly. The current diagnostic tools available require culture and isolation, which can extend the reporting time and delay treatment. Using comparative genomics, we developed an efficient multiplex real-time PCR for the simultaneous detection of all known species of Elizabethkingia, as well as differentiating the two most commonly reported species Elizabethkingia anophelis and Elizabethkingia meningoseptica. |
Haematospirillum jordaniae gen. nov., sp. nov., isolated from human blood samples.
Humrighouse BW , Emery BD , Kelly AJ , Metcalfe MG , Mbizo J , McQuiston JR . Antonie Van Leeuwenhoek 2016 109 (4) 493-500 A Gram-negative, aerobic, motile, spiral-shaped bacterium, strain H5569T, was isolated from a human blood sample. Phenotypic and molecular characteristics of the isolate were investigated. Optimal growth was found to occur at 35 degrees C under aerobic conditions on Heart Infusion Agar supplemented with 5 % rabbit blood. The major fatty acids present in the cells were identified as C16:0, C16:1omega7c and C18:1omega7c. The predominant respiratory quinone was found to be ubiquinone-Q10. The G+C content of genomic DNA for strain H5569T was found to be 49.9 %. Based on 16S rRNA gene sequence analysis results, 13 additional isolates were also analysed in this study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism, represented by strain H5569T, forms a distinct lineage within the family Rhodospirillaceae, closely related to two Novispirillum itersonii subspecies (93.9-94.1 %) and two Caenispirillum sp. (91.2-91.6 %). Based on these results, the isolate H5569T is concluded to represent a new genus and species for which the name Haematospirillum jordaniae gen. nov., sp. nov. is proposed. The type strain is H5569T (=DSMT 28903 = CCUG 66838T). |
Rickettsia parkeri rickettsiosis in different ecological regions of Argentina and its association with Amblyomma tigrinum as a potential vector
Romer Y , Nava S , Govedic F , Cicuttin G , Denison AM , Singleton J , Kelly AJ , Kato CY , Paddock CD . Am J Trop Med Hyg 2014 91 (6) 1156-60 Rickettsia parkeri, a newly recognized tick-borne pathogen of humans in the Americas, is a confirmed cause of spotted fever rickettsiosis in Argentina. Until recently, almost all cases of R. parkeri rickettsiosis in Argentina have originated from the Parana River Delta, where entomological surveys have identified populations of R. parkeri-infected Amblyomma triste ticks. In this report, we describe confirmed cases of R. parkeri rickettsiosis in patients from Cordoba and La Rioja provinces, which are located several hundred kilometers inland, and patients from a more arid ecological region, where A. triste ticks do not occur. Additionally, we identified questing A. tigrinum ticks naturally infected with R. parkeri in Cordoba province. These data provide evidence that another human-biting tick species serves as a potential vector of R. parkeri in Argentina and possibly, other countries of South America. |
Presence and persistence of Coxiella burnetii in the environments of goat farms associated with a Q fever outbreak
Kersh GJ , Fitzpatrick KA , Self JS , Priestley RA , Kelly AJ , Lash RR , Marsden-Haug N , Nett RJ , Bjork A , Massung RF , Anderson AD . Appl Environ Microbiol 2013 79 (5) 1697-703 Q fever is a zoonotic disease caused by inhalation of the bacterium Coxiella burnetii. Ruminant livestock are common reservoirs for C. burnetii, and bacteria present in aerosols derived from the waste of infected animals can infect humans. The significance of infection from material deposited in the environment versus transmission directly from infected animals is not known. In 2011 an outbreak of Q fever cases on farms in Washington and Montana was associated with infected goats. A study was undertaken to investigate the quantity and spatial distribution of C. burnetii in the environment of these goat farms. Soil, vacuum, and sponge samples collected on seven farms epidemiologically linked to the outbreak were tested for the presence of C. burnetii DNA by quantitative PCR. Overall, 70.1% of the samples were positive for C. burnetii. All farms had positive samples, but the quantity of C. burnetii varied widely between samples and between farms. High quantities of C. burnetii DNA were in goat housing/birthing areas, and only small quantities were found in samples collected more than 50 meters from these areas. Follow-up sampling at one of the farms one year after the outbreak found small quantities of C. burnetii DNA in air samples, and high quantities of C. burnetii persisting in soil and vacuum samples. The results suggest that highest concentrations of environmental C. burnetii are found in goat birthing areas and contamination of other areas is mostly associated with human movement. |
A new phlebovirus associated with severe febrile illness in Missouri.
McMullan LK , Folk SM , Kelly AJ , MacNeil A , Goldsmith CS , Metcalfe MG , Batten BC , Albarino CG , Zaki SR , Rollin PE , Nicholson WL , Nichol ST . N Engl J Med 2012 367 (9) 834-41 Two men from northwestern Missouri independently presented to a medical facility with fever, fatigue, diarrhea, thrombocytopenia, and leukopenia, and both had been bitten by ticks 5 to 7 days before the onset of illness. Ehrlichia chaffeensis was suspected as the causal agent but was not found on serologic analysis, polymerase-chain-reaction (PCR) assay, or cell culture. Electron microscopy revealed viruses consistent with members of the Bunyaviridae family. Next-generation sequencing and phylogenetic analysis identified the viruses as novel members of the phlebovirus genus. Although Koch's postulates have not been completely fulfilled, we believe that this phlebovirus, which is novel in the Americas, is the cause of this clinical syndrome. |
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